metabarcoding

The mycorrhizal communities of Lophomyrtus bullata Burret (Myrtaceae) within three natural forest associations of New Zealand

The widespread endemic tree Lophomyrtus bullata (ramarama; Myrtaceae) is in serious decline. Lophomyrtus bullata is now considered threatened due to the ongoing spread of Austropuccinia psidii, a rust fungus causing myrtle rust disease. Mycorrhizal communities play an important role in the survival of plant species and have a potential role in disease resistance. Thus, we examined the fungal communities of L.

Methods for the extraction, storage, amplification and sequencing of DNA from environmental samples

Advances in the sequencing of DNA extracted from media such as soil and water offer huge opportunities for biodiversity monitoring and assessment, particularly where the collection or identification of whole organisms is impractical. However, there are myriad methods for the extraction, storage, amplification and sequencing of DNA from environmental samples.