Nanopore sequencing of metagenomic DNA from rat stomach contents to infer diet

Accurate determination of animal diets is difficult. Methods such as molecular barcoding or metagenomics offer a promising approach allowing quantitative and sensitive detection of different taxa. Here we show that rapid and inexpensive quantification of animal, plant, and fungal content from stomach contents is possible through metagenomic sequencing with the portable Oxford Nanopore Technologies (ONT) MinION. Using an amplification-free approach, we profile the stomach contents from 24 wild-caught rats.

Time-lapse cameras improve our understanding of invertebrate activity in the alpine zone

Understanding when a species is active in its’ environment is essential when designing inventory and monitoring protocols, especially for ectotherms whose activity depends on local weather conditions. The New Zealand alpine zone hosts a diverse native assemblage of invertebrates that are poorly understood yet likely to face an increasing number of threats, particularly associated with climate change and the range expansion of introduced pests.

The pattern of declines and local extinctions of endemic inland Lepidium species in the eastern South Island

Small patches of habitat for indigenous species that remain in developed landscapes are predicted to lose species over time as extinction debts are paid off and to become transformed by spillover from intensive land uses. In December 2020 we searched for plants of three inland South Island-endemic species of Lepidium (Brassicaceae; L. kirkii, L. sisymbrioides, and L. solandri) at previously known locations in Central Otago, the Waitaki Valley, the Mackenzie Basin, and Kura Tāwhiti (Castle Hill, North Canterbury).

Influence of weather on long-tailed bat detection in a North Island exotic forest

Accurate surveys and monitoring are required to guide the conservation and management of threatened species. Some fauna species that are cryptic or difficult to observe because they are nocturnal, mimic other species, conceal themselves, or can be incredibly hard to survey. Emergence and activity of these species may be related to complex environmental cues including weather and atmospheric conditions. The conservation status of New Zealand’s long-tailed bat (Chalinolobus tuberculatus) is Threatened-Nationally Critical.

The mycorrhizal communities of Lophomyrtus bullata Burret (Myrtaceae) within three natural forest associations of New Zealand

The widespread endemic tree Lophomyrtus bullata (ramarama; Myrtaceae) is in serious decline. Lophomyrtus bullata is now considered threatened due to the ongoing spread of Austropuccinia psidii, a rust fungus causing myrtle rust disease. Mycorrhizal communities play an important role in the survival of plant species and have a potential role in disease resistance. Thus, we examined the fungal communities of L.

Population responses of common lizards inside a predator-free dryland sanctuary

Predator-free sanctuaries can assist the conservation of multiple endemic species, but quantitative evidence of these benefits is often lacking, especially for herpetofauna. We measured population responses of three common lizard species (schist geckos, Woodworthia ‘Central Otago’; McCann’s skinks, Oligosoma maccanni; and southern grass skinks, O. aff. polychroma Clade 5) 1 year before and 5 years after mammalian predators were removed inside a mammal-proof fence in a dry grass/shrubland habitat with abundant schist rock in Central Otago, New Zealand.

The invasion of non-native epiphyte Platycerium bifurcatum in Auckland’s urban forest canopy

New Zealand has been subject to extensive plant introductions since the 1840s leading to c. 2700 naturalised plant taxa including 500 serious environmental weeds. To date, non-native plant invasions in New Zealand have not included epiphytic invasions in forest canopies. Numerous records indicate non-mediated naturalisation of individuals of the non-native epiphytic fern Platycerium bifurcatum have been made; further, recent survey work on urban epiphytes identified a number of juveniles across the Auckland isthmus. To investigate the extent of P.

The effect of aerially applied 1080 on the nesting success and survival of kākā

Kākā (Nestor meridionalis) are vulnerable to predation by stoats (Mustela erminea) and possums (Trichosurus vulpecula), particularly when nesting. Without predator control kākā populations decline. Control of stoats and possums both by trapping and aerial application of the poison 1080 leads to increased kākā nesting success. In recent years, many 1080 poison operations have been undertaken to control ship rat (Rattus rattus) and stoat plagues that occur after beech mast. Kākā do not breed every year but breed in anticipation of heavy fruiting.

Effect of pitfall trap design on internal trap temperature and the implications for live-trapped lizards

The capture of animals in live traps poses inherent risks of heat stress and mortality to trapped individuals. Despite a long history of pitfall trap use in New Zealand for monitoring small lizards, the design of traps and their covers often varies; however, the effects that this has on the internal temperature of the traps is unknown. Poor trap design may increase the risk of stress and mortality if internal temperatures exceed thermal limits.

Translocation of Hamilton’s frog, Leiopelma hamiltoni, to a mainland sanctuary occupied by mice Mus musculus

A two-phase translocation of Hamilton's frog (Leiopelma hamiltoni) into Zealandia Ecosanctuary Te Māra a Tāne, in Wellington, was the first attempt to restore the species to the mainland. All non-native mammals had been eradicated there, but house mice (Mus musculus) re-invaded, providing an opportunity to investigate their impact on L. hamiltoni. In Phase I, 60 frogs were translocated into mouse-proof enclosures over 2006–2007.